With regular applications along the lash line of the upper eyelid, Latisse gradually encourages growth of longer, thicker and darker eyelashes. Latisse isn't meant to be applied to the lower eyelid. For full results, you must use the medication daily for at least two months. Eyelash improvements remain as long as you continue to use the medication. When you stop using Latisse, your eyelashes will eventually return to their original appearance.